CD5 Antikörper (FITC)

Produktnummer ABIN125750

Der Maus Monoklonal Anti-CD5-Antikörper wurde für FACS validiert. Er ist geeignet, CD5 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CD5 Antikörper (FITC) (ABIN125750)

Target: CD5

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: CRIS1

Produktdetails anti-CD5 Antikörper (FITC)

Verwendungszweck: Anti-Hu CD5 FITC

Spezifität: The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

Kreuzreaktivität (Details): Human, Other species Not tested

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: stimulated human leukocytes

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD5 Antikörper (FITC) (ABIN125750)

Guarné, Bravo, Calvo, Lozano, Vives, Fita: "Conformation of the hypervariable region L3 without the key proline residue." in: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, (1996) (PubMed).

Alberola-Ila, Places, Cantrell, Vives, Lozano: "Intracellular events involved in CD5-induced human T cell activation and proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 5, pp. 1287-93, (1992) (PubMed).

Arrizabalaga, Mirapeix, Darnell, Torras, Revert: "Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis." in: Nephron, Vol. 53, Issue 1, pp. 41-9, (1989) (PubMed).

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5

Hintergrund: CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

Gen-ID: 921

UniProt: P06127