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CD5 Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD5 in FACS, WB, IP, ELISA und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.
Produktnummer ABIN125749

Kurzübersicht für CD5 Antikörper (ABIN125749)

Target

Alle CD5 Antikörper anzeigen
CD5

Reaktivität

  • 236
  • 87
  • 60
  • 27
  • 22
  • 19
  • 15
  • 12
  • 12
  • 11
  • 11
  • 11
  • 9
  • 2
  • 1
Human

Wirt

  • 179
  • 87
  • 56
  • 1
  • 1
Maus

Klonalität

  • 246
  • 75
  • 1
Monoklonal

Konjugat

  • 120
  • 37
  • 31
  • 27
  • 16
  • 7
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD5 Antikörper ist unkonjugiert

Applikation

  • 245
  • 107
  • 75
  • 69
  • 63
  • 48
  • 31
  • 28
  • 21
  • 18
  • 13
  • 13
  • 12
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

CRIS1
  • Verwendungszweck

    Anti-Hu CD5 Purified

    Spezifität

    The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

    Kreuzreaktivität (Details)

    Human, Other species Not tested

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    stimulated human leukocytes

    Isotyp

    IgG2a
  • Applikationshinweise

    ELISA: The antibody CRIS1 can be used in the sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32
    Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line.
    Western blotting: Laurylmaltoside lysing buffer, non-reducing conditions, recommended dilution: 1-2 μg/mL.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Guarné, Bravo, Calvo, Lozano, Vives, Fita: "Conformation of the hypervariable region L3 without the key proline residue." in: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, (1996) (PubMed).

    Alberola-Ila, Places, Cantrell, Vives, Lozano: "Intracellular events involved in CD5-induced human T cell activation and proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 5, pp. 1287-93, (1992) (PubMed).

    Arrizabalaga, Mirapeix, Darnell, Torras, Revert: "Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis." in: Nephron, Vol. 53, Issue 1, pp. 41-9, (1989) (PubMed).

  • Target

    CD5

    Andere Bezeichnung

    CD5

    Hintergrund

    CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

    Gen-ID

    921

    UniProt

    P06127
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