GST-Tag Antikörper
Kurzübersicht für GST-Tag Antikörper (ABIN125746)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Anti-GST tag Purified
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Spezifität
- The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
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Kreuzreaktivität (Details)
- Recognizes fusion proteins in all species
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Aufreinigung
- Purified by protein-A affinity chromatography.
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Reinheit
- > 95 % (by SDS-PAGE)
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Immunogen
- GST fusion protein
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Isotyp
- IgG2b
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Applikationshinweise
- Western blotting: Recommended dilution: 1 μg/mL, positive control: GST fusion protein, reducing conditions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Do not freeze.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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- GST-Tag
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Andere Bezeichnung
- GST tag
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Substanzklasse
- Tag
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Hintergrund
- The GST (glutathione-S-transferase) is a 26 kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
Target
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