Transferrin Antikörper

Produktnummer ABIN125745

Zitiert in 1 Publikation. Der Maus Monoklonal anti-Transferrin Antikörper (Klon PTF-02) (ABIN125745) detektiert spezifisch Transferrin in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Schwein.

Kurzübersicht für Transferrin Antikörper (ABIN125745)

Target: Transferrin (TF)

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: PTF-02

Produktdetails anti-Transferrin Antikörper

Verwendungszweck: Anti-Por Transferrin Purified

Keine Kreuzreaktivität: Pferd, Schaf

Kreuzreaktivität (Details): Porcine

Produktmerkmale: The antibody PTF-02 recognizes porcine serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Porcine transferrin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Transferrin Antikörper (ABIN125745)

Trebichavský, Mack?, Viklický, Koukal, Siman: "Monoclonal antibodies against pig transferrin. Blocking and binding activity." in: Folia microbiologica, Vol. 32, Issue 5, pp. 448-52, (1988) (PubMed).

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: Transferrin

Hintergrund: Transferrin,Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.,TFQTL1, PRO1557, PRO2086

Gen-ID: 396996

UniProt: P09571

Pathways: Transition Metal Ion Homeostasis