GRM7 Antikörper (Extracellular Domain, N-Term)

Produktnummer ABIN122636

Der Kaninchen Polyklonal anti-GRM7 Antikörper wird verwendet zum Nachweis von GRM7 in Proben von Human, Ratte und Maus. Er wurde validiert für IHC (p).

Kurzübersicht für GRM7 Antikörper (Extracellular Domain, N-Term) (ABIN122636)

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRM7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GRM7 Antikörper

Bindungsspezifität: Extracellular Domain, N-Term

Spezifität: This antibody detects mGluR7 / GRM7 at N-term. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM4 (60 %), GRM6 (45 %).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat (Others see 'Immunogen Remarks')Species reactivity (tested):Human

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide - KLH conjugated

Anwendungsinformationen

Applikationshinweise: Western Blot. Immunohistochemistry with formalin fixed paraffin embedded tissues: 7 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu GRM7

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Andere Bezeichnung: mGluR7 / GRM7

Hintergrund: Metabotropic glutamate receptor GRM7 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. GRM7 is highly concentrated at presynaptic terminals and is thought to play an important role in the modulation of glutamatergic synaptic transmission by effecting presynaptic inhibition of glutamate release. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group III includes GRM4, GRM6, GRM7, and GRM8 receptors, which are linked to inhibition of the cyclic AMP signaling cascade. Five mRNA variants of GRM7 have been described, most of the variants are expressed in brain and retina, although there are non-neuronal variants that may have different functional properties. GRM7 has been reported to be expressed in active zones of presynaptic axon terminals in various regions of the brain, eye, and spinal cord.Synonyms: GPRC1G, MGLUR7, Metabotropic glutamate receptor 7

Gen-ID: 2917

UniProt: Q14831

Pathways: Sensory Perception of Sound, cAMP Metabolic Process, Feeding Behaviour