Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch G Protein-Coupled Receptor 12 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.
GPR12
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
Western Blot. Immunohistochemistry with formalin fixed paraffin embedded tissues: 2-4 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
G Protein-Coupled Receptor 12 (GPR12)
Andere Bezeichnung
GPR12
Hintergrund
GPR12 has been identified as a constitutively active Lysophospholipid/Lysosphingolipid receptor. GPR12 expression has been reported primarily in the central nervous system. Rat transcripts have been identified at low levels in testis and pituitary gland. ESTs have been isolated from human brain and eye libraries, as well as mouse brain, genitourinary tract, and liver libraries. Ligand is Sphingosine-1-phosphate (SPP) Caution: GPR12 is distinct from GPR12A, also known as OGR1, in cluster 3870.Synonyms: G-protein coupled receptor 12