CSK Antikörper (AA 330-450)

Produktnummer ABIN120744

Produktdetails anti-CSK Antikörper

Target: CSK (C-Src tyrosine Kinase (CSK))

Bindungsspezifität: AA 330-450

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CSK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Spezifität: The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.

Aufreinigung: Protein A affinity chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450

Klon: CSK-04

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Western Blotting (1:250-1:300. Weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions). Immunochemistry (Positive control: HeLa human cervix carcinoma cell line).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Andere Bezeichnung: CSK (CSK Produkte)

Synonyme: AW212630 antikoerper, C-terminal Src kinase antikoerper, c-src tyrosine kinase antikoerper, CSK, non-receptor tyrosine kinase antikoerper, CSK antikoerper, Csk antikoerper

Hintergrund: C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.Synonyms: C-SRC kinase, Protein-tyrosine kinase CYL, Tyrosine-protein kinase CSK

Gen-ID: 1445

UniProt: P41240

Pathways: T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events