ACRV1 Antikörper

Produktnummer ABIN120713

Der Maus Monoklonal anti-ACRV1 Antikörper wird verwendet zum Nachweis von ACRV1 in Proben von Human und Schwein. Er wurde validiert für WB, IF und FACS.

Kurzübersicht für ACRV1 Antikörper (ABIN120713)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACRV1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: Hs-8

Produktdetails anti-ACRV1 Antikörper

Spezifität: The antibody reacts with intra-acrosomal sperm proteins (220, 50, 34 and 21 kDa). Reacts with Human, Porcine (Pig).

Produktmerkmale: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Aufreinigung: Precipitation methods and size-exclusion chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: 10 μg/mL, membrane permeabilization (acetone) essential. Flow Cytometry. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Andere Bezeichnung: ACRV1

Hintergrund: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2

Gen-ID: 56

UniProt: P26436