alpha Fetoprotein Antikörper

Produktnummer ABIN120681

Der Maus Monoklonal anti-alpha Fetoprotein Antikörper (Klon AFP-01) (ABIN120681) detektiert spezifisch alpha Fetoprotein in WB, IP und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für alpha Fetoprotein Antikörper (ABIN120681)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon: AFP-01

Produktdetails anti-alpha Fetoprotein Antikörper

Spezifität: The antibody reacts with 70 kDa alpha-Fetoprotein (AFP).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Precipitation Methods

Immunogen: Purified alpha-Fetoprotein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with clone AFP-11 (cat. no. ABIN120683). Western Blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both non-reducing and reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Hintergrund: AFP is an oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gen-ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process