NEFM Antikörper

Produktnummer ABIN120651

Dieses Anti-NEFM-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NEFM in WB, IF und IHC (p). Geeignet für Schwein.

Kurzübersicht für NEFM Antikörper (ABIN120651)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: NF-09

Produktdetails anti-NEFM Antikörper

Spezifität: The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on paraffin sections. Immunocytochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Andere Bezeichnung: Neurofilament M (160 kD)

Hintergrund: Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

UniProt: Q4QRK6

Pathways: Brown Fat Cell Differentiation