CD16 Antikörper

Produktnummer ABIN120566

Der Maus Monoklonal Anti-CD16 Antikörper wurde für FACS, IP, WB und Func validiert. Er ist geeignet, CD16 in Proben von Human zu detektieren.

Kurzübersicht für CD16 Antikörper (ABIN120566)

Target: CD16

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Klon: MEM-154

Produktdetails anti-CD16 Antikörper

Spezifität: The antibody reacts with with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). The antibody MEM-154 recognizes a polymorphism of CD16a (NK cells form), it reacts well with granulocyte form (CD16b) of all subjects.

Aufreinigung: Affinity Chromatography on Protein-A

Immunogen: Human granulocytes

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Recommended dilution: 5-10 m g/mL. Positive control: PBL (peripheral blood lymphocytes) Immunoprecipitation. Western Blotting (non reducing conditions). Functional Application (The antibody blocks binding of human IgG to FcgammaRIII).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: TBS, pH ~8.0 containing 15 mM Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16

Hintergrund: CD16 (FcgRIII) is a 50-80 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gen-ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process