ICAM1 Antikörper (FITC)

Produktnummer ABIN120497

Produktdetails anti-ICAM1 Antikörper (FITC)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM1 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend SM286LE for this use.

Kreuzreaktivität (Details): Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Rat Ax cells (a rat HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Klon: 1A29

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (Use 10 μL neat antibody to label 10^6 cells in 100 μL). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (see Ref.3). Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend the use of ABIN182014 for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL IgG

Buffer: PBS, pH 7.2, containing 1 % BSA as stabiliser and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: CD54 / ICAM1 (ICAM1 Produkte)

Substanzklasse: Viral Protein

Hintergrund: CD54 cell surface antigen, also known as the intercellular adhesion molecule-1 (ICAM-1), is a 90 kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

Gen-ID: 25464

NCBI Accession: NP_037099

UniProt: Q00238

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling