CD43 Antikörper

Produktnummer ABIN120446

Der Maus Monoklonal anti-CD43 Antikörper wird verwendet zum Nachweis von CD43 in Proben von Ratte. Er wurde validiert für FACS, IHC (p) und IHC (fro).

Kurzübersicht für CD43 Antikörper (ABIN120446)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: W3-13

Produktdetails anti-CD43 Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Rat.

Aufreinigung: Protein G Chromatography

Immunogen: Rat thymocyte membrane glycoproteins

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL, this product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections and paraffin sections (requires antigen retrieval using heat treatment prior to staining).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.2 with 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43 / Leukosialin

Hintergrund: CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gen-ID: 10116

UniProt: P13838

Pathways: Regulation of Leukocyte Mediated Immunity