CD28 Antikörper (Biotin)

Produktnummer ABIN120437

Der Maus Monoklonal Anti-CD28-Antikörper wurde für FACS validiert. Er ist geeignet, CD28 in Proben von Ratte zu detektieren.

Kurzübersicht für CD28 Antikörper (Biotin) (ABIN120437)

Target: CD28

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: JJ319

Produktdetails anti-CD28 Antikörper (Biotin)

Spezifität: Antibody SM268B recognizes a 90 kDa homeodimeric cell surface glycoprotein (CD28). Results of tissue Distribution by Flow Cytometry Analysis: Rat strain: Fischer Cell concentration: 1x10e6 cells per test Antibody concentration used: 1.0 μg/10e6 cells Isotypic control: Biotin Mouse IgG1 Cell source percentage of cells stained above control Thymus: 25.4 % Splenic T Cells*: 73.2 % *(T cells isolated with a Rat T Cell Recovery Column).

Aufreinigung: Protein G Chromatography

Immunogen: Rat CD28 transfected A20J cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (for details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Haltbarkeit: 6 months

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat alpha/beta and most gamma/delta T cells, as well as on approximately half of all NK cells.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gen-ID: 25660

NCBI Accession: NP_037253

UniProt: P31042

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response