Factor VIII Antikörper

Produktnummer ABIN120341

Der Maus Monoklonal Anti-Factor VIII-Antikörper wurde für WB, RIA und EIA validiert. Er ist geeignet, Factor VIII in Proben von Human und Schwein zu detektieren.

Kurzübersicht für Factor VIII Antikörper (ABIN120341)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Factor VIII Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Klon: RFFVIIIC-8

Produktdetails anti-Factor VIII Antikörper

Spezifität: Clone RFFVIIIC/8 is a very potent coagulation inhibitor. This antibody recognizes an epitope towards the N-terminus of full length Factor VIII. It also recognises the 210 kDa, 90 kDa and 40 kDa cleavage products. This clone does not cross-react with von Willebrand factor.

Keine Kreuzreaktivität: Maus, Hund, Ratte (Rattus)

Kreuzreaktivität (Details): Species reactivity (tested):Human and Pig.

Aufreinigung: Affinity Chromatography on Protein G from tissue culture supernatant

Immunogen: Affinity purified Human Factor VIII

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1:200 - 1:2000. Western blotting: 1:20 - 1:200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Hintergrund: Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and crosslinks to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries.Synonyms: AHF, Antihemophilic factor, F8C, Procoagulant component

Gen-ID: 2157

NCBI Accession: NP_000123

UniProt: P00451