Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MADCAM1 Antikörper

MADCAM1 Reaktivität: Human WB, FACS, EIA Wirt: Maus Monoclonal 314G8 unconjugated
Produktnummer ABIN120238
  • Target Alle MADCAM1 Antikörper anzeigen
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    Reaktivität
    • 45
    • 45
    • 7
    • 1
    Human
    Wirt
    • 47
    • 15
    • 6
    Maus
    Klonalität
    • 47
    • 21
    Monoklonal
    Konjugat
    • 25
    • 6
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MADCAM1 Antikörper ist unkonjugiert
    Applikation
    • 57
    • 34
    • 26
    • 26
    • 16
    • 13
    • 10
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    Spezifität
    This antibody recognises Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). MAdCAM-1 expression has also been reported in the gut lamina propria but Clone 314G8 does not recognise MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human.
    Aufreinigung
    Affinity Chromatography on Protein G.
    Immunogen
    Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
    Klon
    314G8
    Isotyp
    IgG1
    Top Product
    Discover our top product MADCAM1 Primärantikörper
  • Applikationshinweise
    ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa. Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50, This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Olloquequi, Montes, Prats, Rodríguez, Montero, García-Valero, Ferrer: "Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients." in: The European respiratory journal, Vol. 37, Issue 2, pp. 289-98, (2011) (PubMed).

    Leung, Lehnert, Kanwar, Yang, Mon, McNeil, Krissansen: "Bioassay detects soluble MAdCAM-1 in body fluids." in: Immunology and cell biology, Vol. 82, Issue 4, pp. 400-9, (2004) (PubMed).

  • Target
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    Andere Bezeichnung
    MAdCAM1 (MADCAM1 Produkte)
    Synonyme
    MACAM1 antikoerper, AV211525 antikoerper, mucosal vascular addressin cell adhesion molecule 1 antikoerper, MADCAM1 antikoerper, Madcam1 antikoerper
    Hintergrund
    MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
    Gen-ID
    8174
    NCBI Accession
    NP_570116
    UniProt
    Q13477
Sie sind hier:
Kundenservice