CD163 Antikörper

Produktnummer ABIN120083

Der Maus Monoklonal Anti-CD163 Antikörper wurde für WB, FACS, IF, IHC (p), IHC (fro) und EIA validiert. Er ist geeignet, CD163 in Proben von Human, Affe und Schaf zu detektieren.

Kurzübersicht für CD163 Antikörper (ABIN120083)

Target: CD163

Reaktivität: Human, Affe, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD163 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: EDHu-1

Produktdetails anti-CD163 Antikörper

Spezifität: This antibody recognises the CD163 cell surface antigen. Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin to CD163.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Rhesus Monkey, Sheep.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunohistochemistry on frozen sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund: CD163 is a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gen-ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7