S100A8/A9 Complex (Calprotectin) Antikörper

Produktnummer ABIN119773

Dieser Maus Monoklonal Antikörper detektiert spezifisch S100A8/A9 Complex (Calprotectin) in FACS, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Schwein, Ratte, Rind (Kuh), Cat, Hund, Meerschweinchen, Affe, Kaninchen und Pferd.

Kurzübersicht für S100A8/A9 Complex (Calprotectin) Antikörper (ABIN119773)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reaktivität: Human, Schwein, Ratte, Rind (Kuh), Cat, Hund, Meerschweinchen, Affe, Kaninchen, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MAC387

Produktdetails anti-S100A8/A9 Complex (Calprotectin) Antikörper

Spezifität: This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 12 kDa beta chain expressed by Granulocytes, Monocytes and by tissue Macrophages. Variable results have been reported for staining brain macrophages and microglia.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Horse, Porcine, Canine, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Feline, Cynomologus monkey, Rhesus monkey and Goat.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Human Monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen sections (1/100-1/200). Immunohistochemistry on Paraffin Embedded Sections (1/100-1/200): requires protein digestion pre-treatment e.g. trypsin, 0.1 % for 10 minutes or antigen retrieval using heat treatment prior to staining, see also Ref.2. Recommended Positive Control Tissue: Human Spleen. Flow Cytometry (1/50-1/100): membrane permeabilisation required, see also Ref.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Andere Bezeichnung: MRP8 / MRP14

Hintergrund: Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).Synonyms: MRP8/14, S100A8, S100A8/A9, S100A9

Pathways: S100 Proteine