CD177 Antikörper

Produktnummer ABIN119765

Der Maus Monoklonal anti-CD177 Antikörper wird verwendet zum Nachweis von CD177 in Proben von Human und Rhesusaffen. Er wurde validiert für WB, IP und FACS.

Kurzübersicht für CD177 Antikörper (ABIN119765)

Target: CD177 (CD177 Molecule (CD177))

Reaktivität: Human, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD177 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Klon: MEM-166

Produktdetails anti-CD177 Antikörper

Spezifität: This antibody recognizes CD177 (Neutrophil Glycoprotein NB1). Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). MEM-166 recognizes CD177 under Non-Reducing Conditions.

Aufreinigung: Affinity Chromatography on Protein G

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (1/50 - 1/100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western blot: Non-reducing conditions. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD177

Target: CD177 (CD177 Molecule (CD177))

Andere Bezeichnung: CD177

Hintergrund: CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97 % of the caucasian population.Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1

Gen-ID: 57126

NCBI Accession: NP_065139

UniProt: Q8N6Q3