MCAM Antikörper (N-Term)

Produktnummer ABIN119671

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MCAM in FACS, IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MCAM Antikörper (N-Term) (ABIN119671)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCAM Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: OJ79c

Produktdetails anti-MCAM Antikörper

Bindungsspezifität: AA 1-5, N-Term

Spezifität: This antibody recognizes the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumor progression.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Recombinant human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen sections. Recommended positive control tissue: Melanoma. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, None, None

Konservierungsmittel: Azide free

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C only.

Details zu MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Andere Bezeichnung: CD146

Hintergrund: CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumour progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Gen-ID: 4162

NCBI Accession: NP_006491

UniProt: P43121