Aquaporin 1 Antikörper (AA 249-269)

Produktnummer ABIN119568

Dieses Anti-Aquaporin 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Aquaporin 1 in WB, IP, IHC (p), IHC (fro) und EIA. Geeignet für Human, Ratte und Maus.

Kurzübersicht für Aquaporin 1 Antikörper (AA 249-269) (ABIN119568)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aquaporin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 1-A5F6

Produktdetails anti-Aquaporin 1 Antikörper

Bindungsspezifität: AA 249-269

Spezifität: This antibody recognises an epitope within the cytoplasmic domain of the water-specific channel Aquaporin 1.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Synthetic peptide corresponding to amino acids 249-269 of Rat Aquaporin 1. Spleen cells from immunised Balb/c mice were fused with the cells of the Sp2/0 Ag14 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation: 1/1000 - 1/10000. Western Blot: 1/1000 - 1/5000. ELISA: 1/1000 - 1/20000. Immunohistochemistry on frozen and paraffin embedded sections: 1/500 - 1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended positive control tissue: Kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Andere Bezeichnung: Aquaporin-1

Hintergrund: Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.Synonyms: AQP-1, AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Gen-ID: 358

NCBI Accession: NP_932766

UniProt: P29972

Pathways: Hormone Transport