CD43 Antikörper

Produktnummer ABIN119530

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD43 in FACS, WB, IHC (p), IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD43 Antikörper (ABIN119530)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DFT-1

Produktdetails anti-CD43 Antikörper

Spezifität: This antibody recognises the CD43 cell surface antigen also known as leukosialin and sialophorin.

Aufreinigung: Affinity Chromatography on Protein G

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43 / Leukosialin

Hintergrund: CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gen-ID: 6693

NCBI Accession: NP_001025459

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity