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CD43 Antikörper

SPN Reaktivität: Human FACS, WB, IHC (p), IP, IHC (fro) Wirt: Maus Monoclonal DFT-1 unconjugated
Produktnummer ABIN119530
  • Target Alle CD43 (SPN) Antikörper anzeigen
    CD43 (SPN) (Sialophorin (SPN))
    Reaktivität
    • 133
    • 33
    • 17
    • 1
    Human
    Wirt
    • 79
    • 73
    • 27
    Maus
    Klonalität
    • 111
    • 64
    • 4
    Monoklonal
    Konjugat
    • 76
    • 17
    • 16
    • 16
    • 10
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD43 Antikörper ist unkonjugiert
    Applikation
    • 107
    • 88
    • 54
    • 44
    • 42
    • 21
    • 18
    • 14
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    This antibody recognises the CD43 cell surface antigen also known as leukosialin and sialophorin.
    Aufreinigung
    Affinity Chromatography on Protein G
    Klon
    DFT-1
    Isotyp
    IgG1
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  • Applikationshinweise
    Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, pH 7.4, 0.09 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD43 (SPN) (Sialophorin (SPN))
    Andere Bezeichnung
    CD43 / Leukosialin (SPN Produkte)
    Hintergrund
    CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin
    Gen-ID
    6693
    NCBI Accession
    NP_001025459
    UniProt
    P16150
    Pathways
    Regulation of Leukocyte Mediated Immunity
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