CD43 Antikörper

Produktnummer ABIN119530

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD43 in FACS, WB, IHC (p), IP und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD43 Antikörper (ABIN119530)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DFT-1

Produktdetails anti-CD43 Antikörper

Spezifität: This antibody recognises the CD43 cell surface antigen also known as leukosialin and sialophorin.

Aufreinigung: Affinity Chromatography on Protein G

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43 / Leukosialin

Hintergrund: CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gen-ID: 6693

NCBI Accession: NP_001025459

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity