CD34 Antikörper

Produktnummer ABIN119262

Dieser Anti-CD34 Antikörper ist ein Ratte Monoklonal Antikörper zur Detektion von CD34 in FACS, IP und IHC (p). Geeignet für Maus.

Kurzübersicht für CD34 Antikörper (ABIN119262)

Target: CD34

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEC14-7

Produktdetails anti-CD34 Antikörper

Spezifität: This antibody recognises the CD34 cell surface antigen, wihich is expressed by endothelial cells and by haematopoietic stem cells. It recognises a Neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.

Aufreinigung: Affinity Chromatography on Protein G from tissue culture supernatant.

Endotoxin-Niveau: Low Endotoxin

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gen-ID: 12490

NCBI Accession: NP_598415

UniProt: Q64314