Poly-ADP-Ribose Antikörper

Produktnummer ABIN118982

Der Maus Monoklonal Anti-Poly-ADP-Ribose-Antikörper wurde für WB, IHC (p), IHC (fro) und IP validiert. Er ist geeignet, Poly-ADP-Ribose in Proben von Human zu detektieren.

Kurzübersicht für Poly-ADP-Ribose Antikörper (ABIN118982)

Target: Poly-ADP-Ribose (PAR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Poly-ADP-Ribose Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Klon: 10H

Produktdetails anti-Poly-ADP-Ribose Antikörper

Kreuzreaktivität (Details): Species reactivity (expected):Broad.
Species reactivity (tested):Human.

Aufreinigung: Purified

Immunogen: Purified poly (ADP-ribose) polymer, 10-50 unit chain length.

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Immunohistology on frozen sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Immunoprecipitation. Western Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Poly-ADP-Ribose

Target: Poly-ADP-Ribose (PAR)

Hintergrund: PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).Synonyms: PADPR