TSH receptor Antikörper

Produktnummer ABIN118959

Der Maus Monoklonal anti-TSH receptor Antikörper (Klon 4C1) (ABIN118959) detektiert spezifisch TSH receptor in WB, FACS, IP, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TSH receptor Antikörper (ABIN118959)

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TSH receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 4C1

Produktdetails anti-TSH receptor Antikörper

Spezifität: Recognizes the Human thyroid stimulating hormone (TSH) receptor binding to an extracellular domain. The epitope has been mapped to aa 379-384 (2).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: External domain of Human TSH receptor Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1/AG4.1 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use 10 μL of 1/100-1/1,000 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections (does not require protein digestion pre- treatment nor antigen retrieval using heat treatment prior to staining of paraffin sections). Histology positive control: Thyroid gland. Immunoprecipitation. Western blotting (1/1,000). According to customer information, this antibody has been used successfully on a peptide microarray (2). Not Suitable for ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu TSH receptor

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Andere Bezeichnung: Thyrotropin Receptor / TSHR

Hintergrund: Thyrotropin Receptor, a Glycoprotein Hormone Receptor, plays a central role in controlling thyroid cell metabolism. The TSH receptor can interact with both TSH and autoantibodies against the TSH receptor (thyroid-stimulating immunoglobulins, TSI). Mutations in the receptor are the cause for hyperthyroidism such as gestational hyperthyroidism (hypersensitivity to chorionic gonadotropin) and Grave's disease (autoimmune hyperthyroidism). Multiple isoforms of the thyrotropin receptor are produced by altervative splicing. The thyrotropin receptor has been reported in adipose, adrenal, brain, eye, heart, kidney, skin, thymus, and thyroid. ESTs have been isolated from brain, placenta, and thyroid libraries.Synonyms: LGR3, TSH-R, TSH-receptor, Thyroid-stimulating hormone receptor

Gen-ID: 7253

NCBI Accession: NP_000360

UniProt: P16473

Pathways: Thyroid Hormone Synthesis