P-Selectin Antikörper

Produktnummer ABIN118767

Dieses Anti-P-Selectin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von P-Selectin in WB, FACS, IHC (fro) und IHC (p). Geeignet für Human und Affe.

Kurzübersicht für P-Selectin Antikörper (ABIN118767)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P-Selectin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AK-6

Produktdetails anti-P-Selectin Antikörper

Spezifität: This antibody recognizes CD62P. It reacts with a 140 kDa antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Human platelet membrane glycoproteins.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/1000-1/5000) and Paraffin Embedded Sections (1/500-1/2000): This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH 6.0. Recommended Positive Control Tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the product undiluted at 2-8 °C for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.

Details zu P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Andere Bezeichnung: CD62P / P-Selectin

Hintergrund: CD62P (P-Selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.Synonyms: CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP

Gen-ID: 6403

NCBI Accession: NP_002996

UniProt: P16109