ITGA6 Antikörper

Produktnummer ABIN118734

Produktdetails anti-ITGA6 Antikörper

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGA6 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody recognises the VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: a6b4 integrin purified from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Klon: 450-30A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Andere Bezeichnung: CD49f / ITGA6 (ITGA6 Produkte)

Hintergrund: Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis. CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6

Gen-ID: 3655

NCBI Accession: NP_000201

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex