CD28 Antikörper

Produktnummer ABIN118582

Dieser Ratte Monoklonal Antikörper erkennt spezifisch CD28 in FACS, Func und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD28 Antikörper (ABIN118582)

Target: CD28

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: YTH913-12

Produktdetails anti-CD28 Antikörper

Spezifität: This antibody recognises the CD28 cell surface antigen, a 44 kDa glycoprotein expressed by T lymphocytes. The antibody enhances the MLR response. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.

Aufreinigung: Affinity chromatography on Protein G

Endotoxin-Niveau: Low Endotoxin

Immunogen: Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/50 - 1/100, Use 10 mLof the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Functional Assays: Stimulation of MLR response.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gen-ID: 940

NCBI Accession: NP_006130

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response