MHC Class II RT-1B Antikörper

Produktnummer ABIN118334

Der Maus Monoklonal anti-MHC Class II RT-1B Antikörper wird verwendet zum Nachweis von MHC Class II RT-1B in Proben von Ratte. Er wurde validiert für FACS, IHC (fro), IHC (p) und WB.

Kurzübersicht für MHC Class II RT-1B Antikörper (ABIN118334)

Target: MHC Class II RT-1B

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II RT-1B Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Klon: OX-6

Produktdetails anti-MHC Class II RT-1B Antikörper

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemsitry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MHC Class II RT-1B

Target: MHC Class II RT-1B

Andere Bezeichnung: MHC Class II RT1B

Hintergrund: Synonyms: HLA Class II, MHC Class 2 RT1B