MHC Class II (RT1Bu) Antikörper

Produktnummer ABIN118323

Produktdetails

Target: MHC Class II (RT1Bu)

Reaktivität: Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Affinity chromatography

Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Klon: OX-3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on Lewis rat splenocytes). Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: MHC Class II (RT1Bu)

Andere Bezeichnung: MHC Class II RT1Bu

Gen-ID: 10116

UniProt: Q70RH8