MHC Class II (RT1Bu) Antikörper
Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in FACS, IHC (fro) und IHC (p). Geeignet für Ratte und Maus.
Kurzübersicht für MHC Class II (RT1Bu) Antikörper (ABIN118323)
Target
MHC Class II (RT1Bu)
Reaktivität
Ratte, Maus
Wirt
Maus
Klonalität
Monoklonal
Konjugat
Unkonjugiert
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Klon
OX-3
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Aufreinigung
- Affinity chromatography
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Immunogen
- Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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Isotyp
- IgG1
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Applikationshinweise
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Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on Lewis rat splenocytes). Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II (RT1Bu)
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Andere Bezeichnung
- MHC Class II RT1Bu
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Gen-ID
- 10116
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UniProt
- Q70RH8
Target
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