CD90 Antikörper

Produktnummer ABIN118259

Der Maus Monoklonal Anti-CD90 Antikörper wurde für FACS, WB, IP und IHC (fro) validiert. Er ist geeignet, CD90 in Proben von Maus, Ratte, Kaninchen und Meerschweinchen zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CD90 Antikörper (ABIN118259)

Target: CD90 (THY1)

Reaktivität: Maus, Ratte, Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD90 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: OX-7

Produktdetails anti-CD90 Antikörper

Spezifität: This antibody recognises Rat and Mouse CD90, also known as Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. (5) This clone has been reported to induce glomerular nephritis in Wistar Rats. (8) Affinity of the Fab' of MRC OX-7: Rat Thy1: 3 x 10e9me1 Mouse Thy1.1: 3 x 10e8m-1 (1).

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Rat Thy 1 antigen. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD90 Antikörper (ABIN118259)

Shi, Liang, Guo, Zhang, Hui, Chang, Tao: "Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." in: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).

Details zu CD90

Target: CD90 (THY1)

Andere Bezeichnung: CD90

Hintergrund: Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gen-ID: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization