CD90 Antikörper
Kurzübersicht für CD90 Antikörper (ABIN118258)
Target
Alle CD90 (THY1) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- This antibody recognizes Rat and Mouse CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. This clone has been reported to induce glomerular nephritis in Wistar Rats.Aff. Const.:The affinity of the Fab' of MRC OX-7 for Rat Thy1 is 3 x 10e9m-1 and for Mouse Thy1.1 is 3 x 10e8m-1 (1)
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Aufreinigung
- Affinity Chromatography on Protein G
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Endotoxin-Niveau
- Low Endotoxin
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Immunogen
- Rat Thy1 antigen. Remarks: Spleen cells from an immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
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Isotyp
- IgG1
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Applikationshinweise
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Flow Cytometry: 1/40 - 1/80, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.2 without preservatives
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Konservierungsmittel
- Without preservative
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CD90 (THY1)
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Andere Bezeichnung
- CD90
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Hintergrund
- Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (Rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
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Gen-ID
- 21838
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NCBI Accession
- NP_033408
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UniProt
- P01831
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Pathways
- Cell-Cell Junction Organization
Target
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