ITGA4 Antikörper (FITC)

Produktnummer ABIN118225

Dieser Ratte Monoklonal Antikörper detektiert spezifisch ITGA4 in FACS. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für ITGA4 Antikörper (FITC) (ABIN118225)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGA4 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: PS-2

Produktdetails anti-ITGA4 Antikörper (FITC)

Spezifität: This antibody recognises murine alpha 4 integrin (CD49d). Clone PS/2 has also been reported to block the binding of CD49d to its ligands. We recommend the use of SM037LE for this purpose.

Aufreinigung: Affinity chromatography on Protein G.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/5-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Andere Bezeichnung: CD49d / ITGA4

Hintergrund: Integrin α4 (also called CD49d) is a ~150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin α4 forms heterodimers with integrins β1 and β7. Integrin α4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin α4 promotes cell migration and inhibits cell spreading and contractility. Integrin α4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin α4 function has become an important target for drug discovery. Phosphorylation of the serine 988 residue on the integrin α4 cytoplasmic tail inhibits its interaction with the focal adhesion complex-associated protein paxillin, and modulates its effects on cell motility and spreading.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gen-ID: 3676

NCBI Accession: NP_000876

UniProt: P13612

Pathways: Integrin Complex