CD8 Antikörper

Produktnummer ABIN118177

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch CD8 in FACS und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für CD8 Antikörper (ABIN118177)

Target: CD8

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD8 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: YTS105-18

Produktdetails anti-CD8 Antikörper

Spezifität: This antibody recognises a non polymorphic epitope on the CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Mouse spleen cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD8

Target: CD8

Andere Bezeichnung: CD8

Hintergrund: CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gen-ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731