CD8 Antikörper

Produktnummer ABIN118133

Der Ratte Monoklonal Anti-CD8-Antikörper wurde für FACS, IF und IHC (fro) validiert. Er ist geeignet, CD8 in Proben von Maus zu detektieren.

Kurzübersicht für CD8 Antikörper (ABIN118133)

Target: CD8

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD8 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: KT15

Produktdetails anti-CD8 Antikörper

Spezifität: This antibody recognizes Ly2.1 and Ly2.2 cells, reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: T cell clone, C6. Remarks: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/100 - 1/200, use 10 μL of the suggested working dilution to label 10^6cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD8

Target: CD8

Andere Bezeichnung: CD8

Hintergrund: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gen-ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731