LIM Domain Binding 1 Protein Antikörper (AA 361-373)
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- Target Alle LIM Domain Binding 1 Protein (LDB1) Antikörper anzeigen
- LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
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Bindungsspezifität
- AA 361-373
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Reaktivität
- Human, Maus, Huhn, Xenopus laevis
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser LIM Domain Binding 1 Protein Antikörper ist unkonjugiert
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Applikation
- Enzyme Immunoassay (EIA)
- Spezifität
- This affinity purified antibody is directed against the mouse LDB1protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100 % homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.
- Aufreinigung
- Immunoaffinity chromatography.
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.
- Top Product
- Discover our top product LDB1 Primärantikörper
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- Applikationshinweise
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This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriatecell lysate or extract. This antibody has been used in a ChIP assay using murineerythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1gene, a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was usedsuccessfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20 g for 10e8 cells for ChIP. ELISA: 1: 2,000-1: 8,000. Western Blot: 1: 500-1: 3,000. Immunofluorescence microscopy.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 2-8 °C.
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- Target
- LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
- Andere Bezeichnung
- LDB1 (LDB1 Produkte)
- Synonyme
- CLIM2 antikoerper, NLI antikoerper, chi antikoerper, clim2 antikoerper, nli antikoerper, xldb1 antikoerper, ldb1 antikoerper, LDB1 antikoerper, ldb4 antikoerper, ldb4b antikoerper, LIM domain binding 1 antikoerper, LIM domain binding 1 L homeolog antikoerper, LIM-domain binding 1b antikoerper, LIM domain binding protein 1 antikoerper, LIM domain binding 1a antikoerper, Ldb1 antikoerper, LDB1 antikoerper, ldb1.L antikoerper, ldb1b antikoerper, xldb1 antikoerper, ldb1a antikoerper
- Hintergrund
- LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.Synonyms: CLIM-2, CLIM2, Carboxyl-terminal LIM domain-binding protein 2, LDB1, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, Nuclear LIM interactor, hLdb1
- Gen-ID
- 16825
- NCBI Accession
- NP_001106879
- UniProt
- P70662
- Pathways
- Stem Cell Maintenance, Chromatin Binding
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