Nestin Antikörper (AA 1484-1500)

Produktnummer ABIN117995

Der Kaninchen Polyklonal anti-Nestin Antikörper wird verwendet zum Nachweis von Nestin in Proben von Human und Affe. Er wurde validiert für WB und EIA.

Kurzübersicht für Nestin Antikörper (AA 1484-1500) (ABIN117995)

Target: Nestin (NES)

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Nestin Antikörper

Bindungsspezifität: AA 1484-1500

Spezifität: This antibody detects Nestin protein.

Kreuzreaktivität (Details): Species reactivity (expected):A BLAST analysis was used to suggest cross reactivity with Nestin protein from Human and Macaque (100 % homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from Rabbit (94 %), Mouse (reactivity shown above), Rat and Hamster (88 %), Bovine and Dog (82 %).
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: A recombinant protein corresponding to amino acids 1484-1500 of Human Nestin protein.

Anwendungsinformationen

Applikationshinweise: This antibody has been tested for use in ELISA and Western blot. Expect a bandapproximately 200-220 kDa in size corresponding to Nestin protein by Western blotting inthe appropriate cell lysate or extract. Recommended Dilutions: ELISA: 1/20,000-1/80,000. Western blot: 1/500-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin

Hintergrund: Nestin is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.Synonyms: NES, Nbla00170

Gen-ID: 10763

NCBI Accession: NP_006608

UniProt: P48681