Cecropin B Antikörper

Produktnummer ABIN117971

Der Kaninchen Polyklonal anti-Cecropin B Antikörper wird verwendet zum Nachweis von Cecropin B in Proben von Bombyx mori. Er wurde validiert für IP, WB und EIA.

Kurzübersicht für Cecropin B Antikörper (ABIN117971)

Target: Cecropin B

Reaktivität: Bombyx mori

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cecropin B Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Cecropin B Antikörper

Spezifität: This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.

Aufreinigung: Immunoaffinity chromatography

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetically produced fulllength cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn- Ile-Arg-Asn-Gly- Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala- Lys-Ala-Leu

Anwendungsinformationen

Applikationshinweise: Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value to study cecropins in transgenic plants. The antibody mayalso recognize naturally occurring cecropin A. Cecropin B has been modified in someinstances by substituting a single amino acid to render the molecule less susceptible toproteolytic cleavage. This reagent is suitable for the detection of cecropin B inImmunoblotting (1: 1000), Immunoprecipitation and ELISA experiments. A single 4400dalton band is detected in protein extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.01 M Potassium Phosphate, 0.075 M Sodium Chloride, pH 7.2 with 50 % (v/v) Glycerol as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Details zu Cecropin B

Target: Cecropin B

Andere Bezeichnung: Cecropin-B

Hintergrund: Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.Synonyms: CECB1, Lepidopteran-A/B

Gen-ID: 693028

NCBI Accession: NP_001037460

UniProt: P04142