Der Ziege Polyklonal anti-Invertase Antikörper (ABIN117966) detektiert spezifisch Invertase in WB, EIA und IP.
Dieser Antikörper reagiert spezifisch mit Proben aus Candida sp..
Kurzübersicht für Invertase Antikörper (ABIN117966)
Target
Invertase
Reaktivität
Candida sp.
Wirt
Ziege
Klonalität
Polyklonal
Konjugat
Dieser Invertase Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
Spezifität
This product was prepared from monospecific antiserum. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Invertase [Candida]. Cross reactivity against Invertase from other sources is unknown.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Invertase [Candida] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 65,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
90.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target
Invertase
Hintergrund
Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta D fructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrial and confectionary applications use yeast invertase to split sucrose into fructose and glucose and also to improve the shelf life of food products.Synonyms: Beta-fructofuranosidase, INV1, Saccharase