Cyanine Dyes Antikörper (Biotin)

Produktnummer ABIN117887

Der Schaf Polyklonal Anti--Antikörper wurde für IF, EIA, FACS und IHC (fro) validiert. Er ist geeignet, in Proben von zu detektieren.

Kurzübersicht für Cyanine Dyes Antikörper (Biotin) (ABIN117887)

Target: Cyanine Dyes

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails

Produktmerkmale: Molar Ratio: 10-20 BAC molecule per sheep IgG molecule.

Aufreinigung: Immunoaffinity chromatography

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assay using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended dilutions: This product has been assayed against 1.0 μg of Cyanine IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 8,000 to 1: 40,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % Sodium Azide as preservative and 10 mg/mL BSA as stabilzer, IgG and Protease free.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Antigendetails

Target: Cyanine Dyes

Substanzklasse: Chemical