CD93 Antikörper

Produktnummer ABIN1177363

Produktdetails anti-CD93 Antikörper

Target: CD93

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD93 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Neutralization (Neut)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Klon: R139

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: This NA/LE format is useful for in vitro functional studies. This antibody has been tested for LAL assay for low endotoxin level and by immunofluorescent staining in flow cytometric analysis to assure specificity and reactivity. Immunofluorescent Staining and Flow Cytometric Analysis: The staining technique and controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgG2b isotype control for assessing the level of background staining on human cells is recommended: use at comparable concentrations to antibody of interest (e.g., ≤ 0.125 µg mAb/1 million cells).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CD93 Antikörper (ABIN1177363)

Danet, Luongo, Butler, Lu, Tenner, Simon, Bonnet: "C1qRp defines a new human stem cell population with hematopoietic and hepatic potential." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10441-5, (2002) (PubMed).

Nepomuceno, Ruiz, Park, Tenner: "C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3583-9, (1999) (PubMed).

Tenner: "C1q receptors: regulating specific functions of phagocytic cells." in: Immunobiology, Vol. 199, Issue 2, pp. 250-64, (1999) (PubMed).

Nepomuceno, Tenner: "C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 4, pp. 1929-35, (1998) (PubMed).

Nepomuceno, Henschen-Edman, Burgess, Tenner: "cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro." in: Immunity, Vol. 6, Issue 2, pp. 119-29, (1997) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Guan, Robinson, Goodman, Tenner: "Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 4005-16, (1994) (PubMed).

Guan, Burgess, Robinson, Goodman, McTigue, Tenner: "Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis." in: The Journal of biological chemistry, Vol. 266, Issue 30, pp. 20345-55, (1991) (PubMed).

Details zu CD93

Target: CD93

Andere Bezeichnung: CDw93 (CD93 Produkte)

Synonyme: 6030404G09Rik antikoerper, AA145088 antikoerper, AA4.1 antikoerper, AW555904 antikoerper, C1qr1 antikoerper, C1qrp antikoerper, Ly68 antikoerper, C1QR1 antikoerper, C1qR(P) antikoerper, C1qRP antikoerper, CDw93 antikoerper, ECSM3 antikoerper, MXRA4 antikoerper, dJ737E23.1 antikoerper, C1qRp antikoerper, CD93 antigen antikoerper, CD93 molecule antikoerper, Cd93 antikoerper, CD93 antikoerper

Hintergrund: The immunogen used to raise R139 antibody was C1q-binding protein preparation derived from U937 cell lysates, as described. Human CDw93 (C1qRp) is a 631 AA, single chain type I membrane glycoprotein expressed on cells of myeloid origin, endothelial cells, and hematopoietic progenitor cells. Human, murine and rat protein sequences have been deduced from cDNA clones and are known to be similar in sequence and organization. CDw93 (C1qRp) binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Multivalent interaction of CDw93 (C1qRp) expressing cells with C1q, MBL, and SPA, induces enhancement of phagocytosis of suboptimally opsonized particles and/or cellular debris. Antibody R139 neutralizes/blocks C1q-mediated enhancement of phagocytosis, as reported. In addition clone R139 is suitable to detect CDw93 (C1qRp) expression on cells by flow cytometry, CDw93 (C1qRp) in cellular lysates by Western blotting or immunoprecipitation. CDw93 (C1qRp) has been reported to define a human stem cell population with hematopoietic and hepatic potential.
Synonyms: C1qRp