S6 (pSer240) Antikörper (Alexa Fluor 488)

Produktnummer ABIN1177168

Produktdetails

Target: S6

Bindungsspezifität: pSer240

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Alexa Fluor 488

Applikation: Intracellular Staining (ICS), BioImaging (BI)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human ribosomal protein S6 Peptide

Klon: N4-41

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Referenzen

Jastrzebski, Hannan, Tchoubrieva, Hannan, Pearson: "Coordinate regulation of ribosome biogenesis and function by the ribosomal protein S6 kinase, a key mediator of mTOR function." in: Growth factors (Chur, Switzerland), Vol. 25, Issue 4, pp. 209-26, (2007) (PubMed).

Gu, Santiago, Mitchell: "Synergy between imatinib and mycophenolic acid in inducing apoptosis in cell lines expressing Bcr-Abl." in: Blood, Vol. 105, Issue 8, pp. 3270-7, (2005) (PubMed).

Lal, Li, Smith, Sassano, Uddin, Parmar, Tallman, Minucci, Hay, Platanias: "Activation of the p70 S6 kinase by all-trans-retinoic acid in acute promyelocytic leukemia cells." in: Blood, Vol. 105, Issue 4, pp. 1669-77, (2005) (PubMed).

Shah, Hunter: "Critical role of T-loop and H-motif phosphorylation in the regulation of S6 kinase 1 by the tuberous sclerosis complex." in: The Journal of biological chemistry, Vol. 279, Issue 20, pp. 20816-23, (2004) (PubMed).

Shah, Ghosh, Hunter: "Mitotic regulation of ribosomal S6 kinase 1 involves Ser/Thr, Pro phosphorylation of consensus and non-consensus sites by Cdc2." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16433-42, (2003) (PubMed).

Kleijn, Proud: "The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes." in: BMC biochemistry, Vol. 3, pp. 11, (2002) (PubMed).

Antigendetails

Target: S6

Hintergrund: Ribosomal protein S6 (~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells. The N4-41 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S240.
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6