S6 (pSer235) Antikörper (Alexa Fluor 488)

Produktnummer ABIN1177165

Produktdetails

Target: S6

Bindungsspezifität: pSer235

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Alexa Fluor 488

Applikation: Intracellular Staining (ICS), BioImaging (BI)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human ribosomal protein S6 Peptide

Klon: N7-548

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Referenzen

Blatt, Herrmann, Mirkina, Hadzijusufovic, Peter, Strommer, Hoermann, Mayerhofer, Hoetzenecker, Klepetko, Ghanim, Marth, Füreder, Wacheck, Valenta, Valent: "The PI3-kinase/mTOR-targeting drug NVP-BEZ235 inhibits growth and IgE-dependent activation of human mast cells and basophils." in: PLoS ONE, Vol. 7, Issue 1, pp. e29925, (2012) (PubMed).

Antigendetails

Target: S6

Hintergrund: Ribosomal protein S6 (~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells. The N7-548 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S235 and S236.
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6