BCAR1 Antikörper (pTyr249) (Alexa Fluor 647)

Produktnummer ABIN1177115

Der Maus Monoklonal Anti-BCAR1-Antikörper wurde für ICS validiert. Er ist geeignet, BCAR1 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für BCAR1 Antikörper (pTyr249) (Alexa Fluor 647) (ABIN1177115)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCAR1 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Klon: J169-757-12-2

Produktdetails anti-BCAR1 Antikörper (Alexa Fluor 647)

Bindungsspezifität: pTyr249

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human p130Cas

Isotyp: IgG2b kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-BCAR1 Antikörper (Alexa Fluor 647) (ABIN1177115)

Goldberg, Alexander, Pellicena, Zhang, Tsuda, Miller: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).

Hinsby, Olsen, Bennett, Mann: "Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 1, pp. 29-36, (2003) (PubMed).

Details zu BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Andere Bezeichnung: p130 Cas

Hintergrund: P47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule. The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling