ElF4E (eIF-4E, CBP, EIF4E) (pSer209) Antikörper (Alexa Fluor 647)

Produktnummer ABIN1177053

Produktdetails

Target: ElF4E (eIF-4E, CBP, EIF4E)

Bindungsspezifität: pSer209

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human eIF4E Peptide

Klon: J77-925

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen

Culjkovic, Topisirovic, Borden: "Controlling gene expression through RNA regulons: the role of the eukaryotic translation initiation factor eIF4E." in: Cell cycle (Georgetown, Tex.), Vol. 6, Issue 1, pp. 65-9, (2007) (PubMed).

Wendel, Silva, Malina, Mills, Zhu, Ueda, Watanabe-Fukunaga, Fukunaga, Teruya-Feldstein, Pelletier, Lowe: "Dissecting eIF4E action in tumorigenesis." in: Genes & development, Vol. 21, Issue 24, pp. 3232-7, (2007) (PubMed).

Grolleau, Kaplan, Hanash, Beretta, Richardson: "Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients." in: The Journal of clinical investigation, Vol. 106, Issue 12, pp. 1561-8, (2001) (PubMed).

Waskiewicz, Flynn, Proud, Cooper: "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2." in: The EMBO journal, Vol. 16, Issue 8, pp. 1909-20, (1997) (PubMed).

Antigendetails

Target: ElF4E (eIF-4E, CBP, EIF4E)

Hintergrund: The eukaryotic translation Initiation Factor 4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5' untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified: Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure, over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype, and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex. The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E.
Synonyms: eIF-4E, CBP, EIF4E1, EIF4EL1, EIF4F, mRNA cap-binding protein