IL-18 Receptor (Beta Chain) Antikörper

Produktnummer ABIN1177000

Dieser Ratte Monoklonal Antikörper detektiert spezifisch IL-18 Receptor (Beta Chain) in Neut. Es zeigt Reaktivität gegenüber Proben von Maus. Es wurde in 8+ Publikationen zitiert.

Kurzübersicht für IL-18 Receptor (Beta Chain) Antikörper (ABIN1177000)

Target: IL-18 Receptor (Beta Chain)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Applikation: Neutralization (Neut)

Klon: TC30-28E3

Produktdetails

Produktmerkmale: Neutralization Activity: Assay Type: Proliferation at 50 pg/ml recombinant mouse IL-18 Indicator cell: 2D6 at 10^5 cells/well Preincubation of antibody and cells = 60 minutes
Activity Range: 50% Neutralization (ND50) at 0.2 - 10 µg/ml
>70% Neutralization at 10 - 100 µg/ml

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse sIL-1R7 fusion protein

Isotyp: IgG2a lambda

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen

Neumann, Martin: "Interleukin-12 upregulates the IL-18Rbeta chain in BALB/c thymocytes." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 21, Issue 8, pp. 635-42, (2001) (PubMed).

Debets, Timans, Churakowa, Zurawski, de Waal Malefyt, Moore, Abrams, OGarra, Bazan, Kastelein: "IL-18 receptors, their role in ligand binding and function: anti-IL-1RAcPL antibody, a potent antagonist of IL-18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 4950-6, (2000) (PubMed).

Hoshino, Tsutsui, Kawai, Takeda, Nakanishi, Takeda, Akira: "Cutting edge: generation of IL-18 receptor-deficient mice: evidence for IL-1 receptor-related protein as an essential IL-18 binding receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5041-4, (1999) (PubMed).

Born, Thomassen, Bird, Sims: "Cloning of a novel receptor subunit, AcPL, required for interleukin-18 signaling." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29445-50, (1998) (PubMed).

Tomura, Maruo, Mu, Zhou, Ahn, Hamaoka, Okamura, Nakanishi, Clark, Kurimoto, Fujiwara: "Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 8, pp. 3759-65, (1998) (PubMed).

Xu, Chan, Leung, Hunter, Schulz, Carter, McInnes, Robinson, Liew: "Selective expression and functions of interleukin 18 receptor on T helper (Th) type 1 but not Th2 cells." in: The Journal of experimental medicine, Vol. 188, Issue 8, pp. 1485-92, (1998) (PubMed).

Yoshimoto, Takeda, Tanaka, Ohkusu, Kashiwamura, Okamura, Akira, Nakanishi: "IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-gamma production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 7, pp. 3400-7, (1998) (PubMed).

Maruo, Ahn, Yu, Tomura, Wysocka, Yamamoto, Kobayashi, Hamaoka, Trinchieri, Fuijiwara: "Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 346-52, (1997) (PubMed).

Antigendetails

Target: IL-18 Receptor (Beta Chain)

Hintergrund: The TC30-28E3 antibody specifically binds to the beta subunit of the mouse Interleukin-18 Receptor complex (IL-18Rbeta). The mouse IL-18R complex is expressed by T cells stimulated with IL-12 alone or cultured with antigen and IL-12 to induce Th1 cells. IL-18R is constitutively expressed by NK cells. Resting mouse T cells and Th2 cells do not express detectable levels of IL-18R. The mouse IL-18R complex consists of the IL-18alpha chain, also known as IL-1 Receptor related protein 1 (IL-1Rrp1 or IL-R5) and the IL-18Rbeta chain also known as IL-1R accessory protein-like (IL-1RAcPL or IL-R7). The cell surface IL-18Ralpha chain binds IL-18 with low affinity. However its interaction with the IL-18Rbeta chain confers high affinity binding for the ligand. The IL-18Rbeta chain is not involved in ligand binding but is responsible for the transduction of biological signals. The immunogen used to generate the TC30-28E3 hybridoma was sIL-1R7 fusion protein.
Synonyms: IL-1RAcPL, IL-R7