TER-119/Erythroid Cells Antikörper

Produktnummer ABIN1176982

Produktdetails

Target: TER-119/Erythroid Cells

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Fetal Liver

Klon: TER-119

Isotyp: IgG2b kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen

Kina, Ikuta, Takayama, Wada, Majumdar, Weissman, Katsura: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, (2000) (PubMed).

Maraskovsky, Brasel, Teepe, Roux, Lyman, Shortman, McKenna: "Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1953-62, (1996) (PubMed).

Ikuta, Kina, MacNeil, Uchida, Peault, Chien, Weissman: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, (1990) (PubMed).

Antigendetails

Target: TER-119/Erythroid Cells

Hintergrund: The TER-119 antibody specifically binds to a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the lineage cocktail used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages.
Synonyms: Lymphocyte antigen 76, Ly76, Ly-76, TER-119, Ter119