CD1d Antikörper

Produktnummer ABIN1176968

Produktdetails anti-CD1d Antikörper

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD1d Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma and mouse L929 cells

Klon: 1B1

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Reported applications include immunoprecipitation, blocking of in vitro antigen-mediated stimulation of NK1+T cells, blocking of in vitro responses of some T cells to CD1d1, (possibly to the beta2m-associated form of CD1d, but not to beta2m-independent CD1d4), and in vivo inhibition of TGF-beta2 production. mAb 3C11 (Cat. no. 559871) has been reported to inhibit responses of some T cells to both beta2m-associated and beta2m-independent forms of CD1d.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CD1d Antikörper (ABIN1176968)

Porcelli, Modlin: "The CD1 system: antigen-presenting molecules for T cell recognition of lipids and glycolipids." in: Annual review of immunology, Vol. 17, pp. 297-329, (1999) (PubMed).

Schofield, McConville, Hansen, Campbell, Fraser-Reid, Grusby, Tachado: "CD1d-restricted immunoglobulin G formation to GPI-anchored antigens mediated by NKT cells." in: Science (New York, N.Y.), Vol. 283, Issue 5399, pp. 225-9, (1999) (PubMed).

Szalay, Ladel, Blum, Brossay, Kronenberg, Kaufmann: "Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6955-8, (1999) (PubMed).

Amano, Baumgarth, Dick, Brossay, Kronenberg, Herzenberg, Strober: "CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 4, pp. 1710-7, (1998) (PubMed).

Roark, Park, Jayawardena, Kavita, Shannon, Bendelac: "CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3121-7, (1998) (PubMed).

Brossay, Jullien, Cardell, Sydora, Burdin, Modlin, Kronenberg: "Mouse CD1 is mainly expressed on hemopoietic-derived cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 3, pp. 1216-24, (1997) (PubMed).

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, (1997) (PubMed).

Sydora, Brossay, Hagenbaugh, Kronenberg, Cheroutre: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, (1996) (PubMed).

Bleicher, Balk, Hagen, Blumberg, Flotte, Terhorst: "Expression of murine CD1 on gastrointestinal epithelium." in: Science (New York, N.Y.), Vol. 250, Issue 4981, pp. 679-82, (1990) (PubMed).

Details zu CD1d

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Andere Bezeichnung: CD1d (CD1D Produkte)

Synonyme: CD1A antikoerper, R3 antikoerper, CD1D antikoerper, CD1D1 antikoerper, CD1d molecule antikoerper, CD1D antigen, d polypeptide antikoerper, CD1D antikoerper

Hintergrund: The 1B1 antibody reacts with CD1d, a 48-kDa glycoprotein with structural homology to the major histocompatibility complex (MHC) class I molecules. The structure, expression, and functions of CD1 antigens are complex and have been reviewed. mAb 1B1 detects CD1d at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus. It appears to recognize CD1d only in association with beta2m. CD1d has been reported to be expressed by gastrointestinal tract epithelium and in the cytoplasm of hepatocytes as detected via immunohistochemical staining of frozen sections with mAb 3C11 (Cat. No. 559871, for the purified antibody), suggesting a possible role for CD1d in mucosal immunity. However, CD1d expression was not detectable, via flow cytometry, on intestinal epithelial cells in studies using the anti-CD1d mAbs 3C11, 1B1, and 9C7. The 1B1 antibody competes with mAb 3C11 in binding to mouse splenocytes.
Synonyms: CD1.1, Ly-38

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process