FBLN3 Antikörper (AA 258-493) (Biotin)

Produktnummer ABIN1176446

Produktdetails anti-FBLN3 Antikörper (Biotin)

Target: FBLN3 (Fibulin 3 (FBLN3))

Bindungsspezifität: AA 258-493

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FBLN3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequenz: MGHHHHHHSG SEF-CDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT SSYLCQYQCV NEPGKFSCMC PQGYQVVRSR TCQDINECET TNECREDEMC WNYHGGFRCY PRNPCQDPYI LTPENRCVCP VSNAMCRELP QSIVYKYMSI RSDRSVPSDI FQIQATTIYA NTINTFRIKS GNENGEFYLR QTSPVSAMLV LVKSLSGPRE HIVDLEMLTV SSIGTFRTSS VLRLTIIVGP FSF

Spezifität: It has been selected for its ability to recognize FBLN3 in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant FBLN3 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against FBLN3 conjugated to biotin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant FBLN3 (Cys258~Phe493) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu FBLN3

Target: FBLN3 (Fibulin 3 (FBLN3))

Abstract: FBLN3 Produkte

Synonyme: DHRD antikoerper, DRAD antikoerper, FBLN3 antikoerper, FBNL antikoerper, FIBL-3 antikoerper, MLVT antikoerper, MTLV antikoerper, S1-5 antikoerper, T16 antikoerper, EGF containing fibulin like extracellular matrix protein 1 antikoerper, EGF-containing fibulin-like extracellular matrix protein 1 antikoerper, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 antikoerper, EFEMP1 antikoerper, Efemp1 antikoerper

Pathways: EGFR Signaling Pathway