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Complement C4c Antikörper (cleaved) (Biotin)

Dieses Anti-Complement C4c-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Complement C4c in WB, IHC und ICC. Geeignet für Human.
Produktnummer ABIN1171857

Kurzübersicht für Complement C4c Antikörper (cleaved) (Biotin) (ABIN1171857)

Target

Complement C4c (C4c) (Complement Component C4c (C4c))

Reaktivität

Human

Wirt

  • 12
  • 4
  • 1
Kaninchen

Klonalität

  • 13
  • 4
Polyklonal

Konjugat

  • 10
  • 3
  • 1
  • 1
  • 1
  • 1
Dieser Complement C4c Antikörper ist konjugiert mit Biotin

Applikation

  • 12
  • 10
  • 7
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 2
    • 1
    cleaved

    Verwendungszweck

    Biotin-Linked Polyclonal Antibody to Complement Component 4c (C4c)

    Spezifität

    The antibody is a rabbit polyclonal antibody raised against C4c. It has been selected for its ability to recognize C4c in immunohistochemical staining and western blotting.

    Aufreinigung

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Native Protein C4cC4b was cleaved by C3b inactivator (C3bINA) in the presence of C4b-binding protein and C4d and C4c isolated. The radioactive label and therefore the reactive thiol group were located to C4d. C4c was reduced and alkylated and the two alpha'-chain fragments of C4c were separated. The molecular weights, amino acid analyses and carbohydrate content of the three alpha'-chain fragments were determined. C4d has a mol.wt. of 44500 and a carbohydrate content of 6 % . The two alpha'-chain fragments of C4c have mol.wts. of 25000 and 12000 and carbohydrate contents of 10 and 22 % respectively.
    The antibody is a rabbit polyclonal antibody raised against C4c conjugated to biotin.

    Isotyp

    IgG
  • Applikationshinweise

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Kommentare

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handhabung

    Avoid repeated freeze/thaw cycles

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    Complement C4c (C4c) (Complement Component C4c (C4c))

    Andere Bezeichnung

    Complement Component 4c
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